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RNA-seq profiling in dorsal root ganglian (DRG) after fracture surgery and Protectin DX treatment

GSE309530 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2025/11/07 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
18 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Protectin DX resolves fracture-induced postoperative pain in mice via neuronal signaling and GPR37-activated macrophage efferocytosisThe Journal of clinical investigation · 2026
Summary (written by the submitters)
This study investigates the molecular mechanisms by which Protectin DX (PDX), a specialized pro-resolving mediator derived from docosahexaenoic acid, alleviates fracture-induced postoperative pain through GPR37 receptor signaling. The bulk RNA-sequencing experiment was conducted on L3-L5 dorsal root ganglia (DRG) tissues collected from CD1 mice three days after tibial fracture surgery, comparing gene expression profiles between PDX-treated and vehicle-treated groups.
Read the full summary ↓
The primary goals are to identify differentially expressed genes regulated by PDX treatment, with particular focus on macrophage/neutrophil signaling, cytokine responses, and neuronal excitability pathways that contribute to pain resolution. This transcriptomic analysis aims to elucidate the molecular basis of PDX's superior analgesic efficacy compared to conventional anti-inflammatory treatments, and to understand how PDX promotes rather than delays the resolution of postoperative pain. The findings have significant clinical relevance for developing safer, non-opioid therapeutic strategies for postoperative pain management, particularly in orthopedic surgery patients, where current treatments often remain inadequate despite multimodal approaches.
Published in
Protectin DX resolves fracture-induced postoperative pain in mice via neuronal signaling and GPR37-activated macrophage efferocytosis
Li Y, Bang S, Ji J et al. · The Journal of clinical investigation 2026 · PMID 41542772 · doi:10.1172/JCI190754
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE309530_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1297118 and SRA study SRP604565. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 18 more — browse all 18 samples with per-sample file links →

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