GEO series

PRMT7-specific PROTAC degrader achieves improved adoptive cancer immunotherapy by enhancing CD8+ T effector function through NF-kB activation.

GSE309747 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/07/01 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 12 samples: 3 × T cells, WT DMSO, vs 3 × T cells, WT PROTAC, vs 3 × T cells, PRMT7 control, vs 3 × T cells, PRMT7 KO,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

New therapeutics are required to ameliorate cytotoxic T lymphocytes (CTL) for improved immunotherapy and T cell therapies. Herein we report that deletion of Prmt7 in T cells in mice with CD4-Cre increased CD8+ T effector cell cytokine production, cytolytic activity and anti-tumor activity of CTLs by transcriptional reprogramming. We show that Prmt7-deficiency in CTLs reprograms the CD8+ T cells with many transcription factors allowing them to exhibit increased proliferation, and express effector molecules.

Read the full summary ↓

To translate these findings into a potential clinically applicable treatment, we generated a PRMT7 PROTAC degrader (MS54). We show that CTLs treated with the MS54 had similar reprogramming as the Prmt7 cKO CTLs. Adoptive cell transfer of MS54-treated CTLs from OT-I mice significantly enhanced their anti-tumor activity in a syngeneic melanoma murine cancer model. Human CTLs treated with the MS54 had increased proliferation, and enhanced activation markers. Our findings show that the MS54 has potential therapeutic utility for immunotherapy and adoptive cell therapy by reprogramming CTLs.

This dataset

Open GSE309747 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE309747_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1336848 and SRA study SRP630186. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

3 × T cells, WT DMSO, vs 3 × T cells, WT PROTAC, vs 3 × T cells, PRMT7 control, vs 3 × T cells, PRMT7 KO,

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9278290 T cells, WT DMSO, replicate 1 T cells, WT DMSO,
GSM9278291 T cells, WT DMSO, replicate 2 T cells, WT DMSO,
GSM9278292 T cells, WT DMSO, replicate 3 T cells, WT DMSO,
GSM9278293 T cells, WT PROTAC, replicate 1 T cells, WT PROTAC,
GSM9278294 T cells, WT PROTAC, replicate 2 T cells, WT PROTAC,
GSM9278295 T cells, WT PROTAC, replicate 3 T cells, WT PROTAC,
GSM9278296 T cells, PRMT7 control, replicate 1 T cells, PRMT7 control,
GSM9278297 T cells, PRMT7 control, replicate 2 T cells, PRMT7 control,
GSM9278298 T cells, PRMT7 control, replicate 3 T cells, PRMT7 control,
GSM9278299 T cells, PRMT7 KO, replicate 1 T cells, PRMT7 KO,
GSM9278300 T cells, PRMT7 KO, replicate 2 T cells, PRMT7 KO,
GSM9278301 T cells, PRMT7 KO, replicate 3 T cells, PRMT7 KO,

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.