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Effect of Recombinant ApoE on Gene Expression During Osteoblast Differentiation of Mouse Bone Marrow Stromal Cells (BMSCs)

GSE309814 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/12/01 Platform GPL34290
Summary
Advanced age impairs bone fracture healing; however, the underlying mechanisms remain unclear. We found that circulating levels of apolipoprotein E (ApoE) increase with age and contribute to impaired fracture healing. In vitro, ApoE treatment of aged bone marrow stromal cells (BMSCs) inhibited osteoblast differentiation. To investigate the mechanism by which ApoE inhibits osteoblast differentiation, we treated primary BMSCs isolated from the femurs and tibiae of 24-month-old C57BL6/J mice with recombinant ApoE. For osteoblast differentiation, BMSCs were cultured in osteogenic medium. Recombinant ApoE (rApoE) was added at a concentration of 100 ng/mL starting two days after induction of differentiation and continued throughout the 10-day differentiation period. BMSCs treated with vehicle or rApoE were harvested on day 10. Total RNA was extracted using TRIzol Reagent (Invitrogen) according to the manufacturer’s protocol for downstream transcriptomic analysis.
Published in
Neutralizing hepatic apolipoprotein E enhances aged bone fracture healing
Huang M, Balu AR, Molitoris KH et al. · Bone research 2026 · PMID 41571629 · doi:10.1038/s41413-025-00489-y
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Also filed as BioProject PRJNA1363759 and SRA study SRP644428. Searching any of these in the dataset finder brings you back here.

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