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ScRNAseq analysis of data from patients with T1D and healthy donors

GSE309970 Homo sapiens Expression profiling by high throughput sequencing 5 samples Submitted 2025/11/15 Platform GPL24676
Summary
Type 1 diabetes (T1D) arises from loss of immune tolerance to pancreatic β-cells, yet its immunologic drivers remain unclear. We performed single-cell transcriptomic profiling of peripheral T cells from children newly diagnosed with T1D, the same children one year later, and healthy controls. At diagnosis, T1D was marked by broadly reduced effector and cytotoxic programs and increased stemness-related signatures across multiple T cell subsets, alongside impaired regulatory features in both Tregs and TR3-56 cells. Flow cytometry from the same cohort and reanalysis of public datasets confirmed these patterns. Together, these data suggest that T1D involves defective T cell effector differentiation and compromised regulatory control, contributing to immune dysregulation and loss of self-tolerance.
Published in
Imbalance of stem-like and effector T cell states in children with early type 1 diabetes across conventional and regulatory subsets
Niederlova V, Neuwirth A, Neuman V et al. · Nature communications 2025 · PMID 41381478 · doi:10.1038/s41467-025-66459-4
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Also filed as BioProject PRJNA1364068. Searching any of these in the dataset finder brings you back here.

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