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Reprogramming lineage-traced Müller glia for robust proliferation and neurogenesis in adult mammalian retinas

GSE310001 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/07/21 Platform GPL24247
Summary
In cold-blooded vertebrates such as zebrafish, Müller glia (MG) act as endogenous retinal stem/progenitor cells upon retinal injury, which evokes a regenerative response that proceeds first with an symmetric division to generate a MG-derived multipotent progenitor cell robust proliferation of MG-derived multipotent progenitors (MGPCs) through that undergoes multiple rounds of cell division, followed by differentiation of MGPCs into new retinal neurons. By contrast, mammalian MG undergo reactive gliosis following retinal injury rather than cell-cycle re-entry for proliferation, hindering its capacity to replenish the lost retinal neuronal cells under pathological conditions. In this study, we treat the adult mouse retina with the combination of FGF2 (Fibroblast growth factor 2) and RA (retinoic acid) after AAV-mediated MG-specific gene transfer of Ascl1 (FAR), and scRNA-seq was employed to see whether the FAR treatment could induced robust MG proliferation and neural regeneration in adult mouse retina.
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Direct links to NCBI, no account and no request form: the whole study as GSE310001_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1364053 and SRA study SRP645390. Searching any of these in the dataset finder brings you back here.

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