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Sulforaphane Inhibits Colorectal Cancer Progression via PI3K/AKT/mTOR and HIF-1α Suppression with Enhanced Efficacy under Hypoxia

GSE310085 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2025/12/01 Platform GPL34284
Summary
Sulforaphane (SFN) exhibits anticancer activity across diverse tumor models, yet its role under hypoxia—a hallmark of the colorectal cancer microenvironment—remains incompletely defined. In this study, colorectal cancer cells were cultured under hypoxic conditions and treated with SFN or a combination of SFN and 5-fluorouracil (5-FU). RNA-seq profiling was performed to characterize transcriptional alterations associated with SFN activity and SFN-mediated reversal of hypoxia-induced 5-FU resistance. The dataset includes paired-end raw FASTQ files, supporting further investigation into hypoxia-related signaling networks, including PI3K/AKT/mTOR and HIF-1α pathways.
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Direct links to NCBI, no account and no request form: the whole study as GSE310085_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1365101 and SRA study SRP645173. Searching any of these in the dataset finder brings you back here.

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