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Dynamic Resilience: Stress Phenotypes and Their Impact on Opioid-Taking Behavior, Brain Activity, and Gene Expression

GSE310244 Mus musculus (mouse) RNA-seq (gene expression) 68 samples Submitted 2026/07/01 Platform GPL34290
At a glance

RNA-seq (gene expression) · mouse · 68 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
68 samples: 34 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Stress is a common experience that reveals individual differences in vulnerability. While some individuals succumb to lasting maladaptive behaviors, such as substance use disorders, others demonstrate resilience—a dynamic ability to return to baseline functioning over time. Assessing stress-related behaviors at a single time point may overlook key aspects of resilience.
Read the full summary ↓
We used chronic social defeat stress (CSDS) to examine phenotypic stability and found mice distributed into the expected susceptible/resilient ratio at both 24 hours and three weeks, with most maintaining their phenotype over the three-week period. We then evaluated intravenous self-administration (IVSA) of remifentanil. One-week post-CSDS, all stressed mice showed increased opioid intake, regardless of phenotype. However, by three weeks, intake in resilient mice returned to baseline levels, while intake in susceptible mice remained elevated. To examine neural correlates of these behavioral shifts, we measured cFOS activity in the basolateral amygdala (BLA) and nucleus accumbens (NAc). In susceptible mice, BLA cFOS remained elevated at both time points. NAc activity was elevated in both groups at one week but normalized in resilient mice by three weeks. This pattern paralleled opioid intake behavior. Additionally, we found distinct gene expression changes that may underlie the adaptive shifts seen in resilient mice. These findings underscore that resilience is a dynamic process characterized by evolving behavioral and neural responses. By evaluating multiple time points, our research provides new insights into how different stress phenotypes influence opioid use trajectories and highlights critical brain regions involved in these processes.
This dataset
Open GSE310244 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE310244_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1365522 and SRA study SRP646117. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
34 conditions, mostly in duplicate
1-week, Susceptible, mouse 1, ×2 1-week, Susceptible, mouse 2, ×2 1-week, Susceptible, mouse 3, ×2 1-week, Susceptible, mouse 4, ×2 1-week, Susceptible, mouse 5, ×2 1-week, Resilient, mouse 1, ×2 1-week, Resilient, mouse 2, ×2 1-week, Resilient, mouse 3, ×2 +26 more

Supports a between-group comparison across 68 samples.

How we read this: 34 replicated groups read from 68 sample titles; they account for 68 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 28 more — browse all 68 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.