← BioTransfer GEO Dataset Finder
GEO series

Distinct contributions of Etv2+ and Flk1+ progenitors to endothelial, hematopoietic, and cardiac lineages [RNA-seq]

GSE310548 Mus musculus (mouse) RNA-seq (gene expression) 21 samples Submitted 2025/11/21 Platform GPL21103
At a glance

RNA-seq (gene expression) · mouse · 21 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
21 samples: 7 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Distinct contributions of Etv2(+) and Flk1(+) progenitors to endothelial, hematopoietic, and cardiac lineagesCell reports · 2026
Summary (written by the submitters)
The ETS family transcription factor ETV2, VEGFA and its receptor FLK1 are essential for hematopoietic, vascular and cardiac development. Here, we combine dual Etv2 and Flk1 lineage tracing with molecular profiling to define how mesoderm progenitors are allocated to hematopoietic, endothelial, cardiomyocyte and smooth muscle lineages. We demonstrate that hematopoietic, endothelial, and cardiac valves arise from dual Etv2+ and Flk1+ lineages and that Etv2+ and Flk1+ mesoderm contributing to the hemangiogenic fate is molecularly distinct from those generating muscle.
Read the full summary ↓
Mechanistically, we show that ETV2 cooperates with the BAF chromatin remodeling complex to establish accessibility at ETV2 target loci. Loss of Baf155 expression reduces chromatin accessibility at ETV2 target loci and impairs hemangiogenic lineage specification. This work defines lineage relationships and the molecular circuitry underlying hemangiogenic specification during cardiovascular development.
Published in
Distinct contributions of Etv2(+) and Flk1(+) progenitors to endothelial, hematopoietic, and cardiac lineages
Alleyne D, Kim M, Wu J et al. · Cell reports 2026 · PMID 41420864 · doi:10.1016/j.celrep.2025.116733
This dataset
Open GSE310548 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE310548_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1366501 and SRA study SRP646979. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41420864) ↗
Study design
7 conditions, mostly in triplicate
TGET EBs, PDGFRalpha+FLK1+, Etv2-tdTomato neg… ×3 TGET EBs, PDGFRalpha+FLK1+, Etv2-tdTomato pos… ×3 TGET EBs, PDGFRalpha-FLK1+, Etv2-tdTomato neg… ×3 TGET EBs, PDGFRalpha-FLK1+, Etv2-tdTomato pos… ×3 Wildtype EBs, PDGFRalpha+FLK1+, ×3 Wildtype EBs, PDGFRalpha-FLK1+, ×3 iETV2 EBs, PDGFRalpha-FLK1+, ×3

Supports a between-group comparison across 21 samples.

How we read this: 7 replicated groups read from 21 sample titles; they account for 21 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.