Decoding the Cell-Type and Spatial Roles of H19 in Cholestatic Liver Injury Through Single-Nucleus and Spatial Transcriptomics

Despite recent advances, Primary Sclerosing Cholangitis (PSC)-a chronic obstructive biliary disease-still lacks effective therapies to prevent disease progression or the need for liver transplantation. Long non-coding RNA H19 (H19) has been implicated in promoting PSC disease progression. However, the underlying cell-specific and molecular mechanisms by which H19 contributes to PSC pathogenesis remain incompletely understood. Here we used single nucleus RNA sequencing (snRNAseq) and spatial transcriptomics to elucidate the cell- and spatial-specific expression alterations associated with H19 in the Mdr2KO PSC mouse model.