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Age- and Sex- Driven Transcriptional and Metabolic Diversity in Myeloid-Derived Suppressor Cells After Mouse Sepsis

GSE311155 Mus musculus (mouse) Single-cell RNA-seq 57 samples Submitted 2026/04/15 Platform GPL34290
At a glance

Single-cell RNA-seq · mouse · 57 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism
Mus musculus (mouse)
Samples
57 samples: 17 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
A murine model of sepsis induces age- and sex-specific chromatin remodeling in myeloid-derived suppressor cellsFrontiers in immunology · 2026
Summary (written by the submitters)
Sepsis induces profound immune dysregulation, often resulting in chronic critical illness characterized by persistent immunosuppression and poor outcomes. Myeloid-derived suppressor cells (MDSCs) are central mediators of this immunosuppressive phenotype, yet the influence of age and sex on their transcriptional and metabolic states remain poorly understood.
Read the full summary ↓
Here, we employed single-cell RNA sequencing of splenic leukocytes from young (3-4 months) and older (18-24 months) adult male and female mice subjected to a clinically relevant murine sepsis model to define age- and sex-specific MDSC phenotypes. We identified significant differences regarding age and sex in MDSC expansion, transcriptome, canonical pathway activation, RNA velocity, mitochondrial metabolism, and predicted cell-cell communication after sepsis. Using drug2cell analysis of total leukocytes we also identified cohort-specific drug target profiles. These findings underscore the importance of age and sex in shaping sepsis-induced MDSC biology and suggest that personalized immunomodulatory strategies targeting MDSCs could improve sepsis outcomes.
Published in
A murine model of sepsis induces age- and sex-specific chromatin remodeling in myeloid-derived suppressor cells
Charles AM, Rodhouse CE, Darden DB et al. · Frontiers in immunology 2026 · PMID 41953016 · doi:10.3389/fimmu.2026.1750174
This dataset
Open GSE311155 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE311155_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1368753 and SRA study SRP648235. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41953016) ↗
Study design
17 conditions, mostly in duplicate
Mus musculus, splenocytes, 18-24 months, Male… ×5 Mus musculus, splenocytes, 3-4 months, Female… ×4 Mus musculus, splenocytes, 3-4 months, Male, … ×4 Mus musculus, splenocytes, 3-4 months, Female… ×4 Mus musculus, splenocytes, 3-4 months, Male, … ×4 Mus musculus, splenocytes, 18-24 months, Fema… ×3 Mus musculus, splenocytes, 18-24 months, Male… ×3 Mus musculus, splenocytes, 18-24 months, Fema… ×3 +18 more

Supports a between-group comparison across 48 samples.

How we read this: 17 replicated groups read from 57 sample titles; they account for 48 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 17 more — browse all 57 samples with per-sample file links →

Datasets on a similar topic

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