GEO series

MEK inhibition induces AXIN1 loss in colorectal cancer by mTOR associated suppression of protein synthesis [RNASeq_SW480]

GSE312195 Homo sapiens (human) RNA-seq (gene expression) 27 samples Submitted 2026/06/03 Platform GPL24676

At a glance

RNA-seq (gene expression) · human · 27 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Samples: 27 samples: 9 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Ras-MAPK inhibition induces AXIN1 loss in colorectal cancer by mTOR associated suppression of protein synthesisCell communication and signaling : CCS · 2026

Summary (written by the submitters)

AXIN1 is a central regulatory hub of many oncogenic pathways in colorectal cancer (CRC). As the main scaffold protein and least abundant component of the beta-catenin destruction complex, changes in AXIN1 levels affect Wnt signaling output. We show that targeting the Ras-MAPK pathway by MEK1/2 inhibitors induces AXIN1 loss across a panel of CRC cell lines and patient-derived organoids.

Read the full summary ↓

GSK3B inhibition similarly reduced AXIN1 levels, yet by distinct mechanisms. MEK1/2 causes a reduction of AXIN1 transcript levels, but neither affects protein stability nor post-translational modifications of AXIN1. In contrast, GSK3B inhibition induces rapid AXIN1 degradation. Prevention of AXIN1 loss by co-treatment with tankyrase inhibitors was much stronger for GSK3B than for MEK1/2 inhibition. Using isogenic CRC cell lines and murine intestinal organoids, we show that APC truncations strongly reduce basal AXIN1 levels, but do not alter dynamics of AXIN1 loss upon MEK1/2 inhibition. Polysome profiling and Ribo-Seq revealed that MEK1/2 inhibition reduces global protein synthesis via an mTOR dependent pathway. This translational repression is sufficient to cause significant AXIN1 loss, as treatment with mTOR inhibitors phenocopies the effect of MEK1/2 inhibitors. Our study demonstrates that AXIN1 protein homeostasis is critically controlled by Ras-MAPK signaling at the level of protein synthesis, and that MEK1/2 inhibitors cause AXIN1 loss by translational repression.

Published in

Ras-MAPK inhibition induces AXIN1 loss in colorectal cancer by mTOR associated suppression of protein synthesis

Venkatachalam N, Wang L, Muthukumarana N et al. · Cell communication and signaling : CCS 2026 · PMID 42204589 · doi:10.1186/s12964-026-02963-4

This dataset

Open GSE312195 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE312195_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1372651 and SRA study SRP650668. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42204589) ↗

Study design

9 conditions, mostly in triplicate

SW480, Chir, 24h, ×3 SW480, Chir, 72h, ×3 SW480, Chir, 8h, ×3 SW480, DMSO, 24h, ×3 SW480, DMSO, 72h, ×3 SW480, DMSO, 8h, ×3 SW480, Tram, 24h, ×3 SW480, Tram, 72h, ×3 +1 more

Supports a between-group comparison across 27 samples.

How we read this: 9 replicated groups read from 27 sample titles; they account for 27 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9340215 SW480, Chir, 24h, rep1 SW480, Chir, 24h,
GSM9340216 SW480, Chir, 72h, rep1 SW480, Chir, 72h,
GSM9340217 SW480, Chir, 8h, rep1 SW480, Chir, 8h,
GSM9340218 SW480, Chir, 24h, rep2 SW480, Chir, 24h,
GSM9340219 SW480, Chir, 72h, rep2 SW480, Chir, 72h,
GSM9340220 SW480, Chir, 8h, rep2 SW480, Chir, 8h,
GSM9340221 SW480, Chir, 24h, rep3 SW480, Chir, 24h,
GSM9340222 SW480, Chir, 72h, rep3 SW480, Chir, 72h,
GSM9340223 SW480, Chir, 8h, rep3 SW480, Chir, 8h,
GSM9340224 SW480, DMSO, 24h, rep1 SW480, DMSO, 24h,
GSM9340225 SW480, DMSO, 72h, rep1 SW480, DMSO, 72h,
GSM9340226 SW480, DMSO, 8h, rep1 SW480, DMSO, 8h,
GSM9340227 SW480, DMSO, 24h, rep2 SW480, DMSO, 24h,
GSM9340228 SW480, DMSO, 72h, rep2 SW480, DMSO, 72h,
GSM9340229 SW480, DMSO, 8h, rep2 SW480, DMSO, 8h,
GSM9340230 SW480, DMSO, 24h, rep3 SW480, DMSO, 24h,
GSM9340231 SW480, DMSO, 72h, rep3 SW480, DMSO, 72h,
GSM9340232 SW480, DMSO, 8h, rep3 SW480, DMSO, 8h,
GSM9340233 SW480, Tram, 24h, rep1 SW480, Tram, 24h,
GSM9340234 SW480, Tram, 72h, rep1 SW480, Tram, 72h,
GSM9340235 SW480, Tram, 8h, rep1 SW480, Tram, 8h,
GSM9340236 SW480, Tram, 24h, rep2 SW480, Tram, 24h,
GSM9340237 SW480, Tram, 72h, rep2 SW480, Tram, 72h,
GSM9340238 SW480, Tram, 8h, rep2 SW480, Tram, 8h,
GSM9340239 SW480, Tram, 24h, rep3 SW480, Tram, 24h,
GSM9340240 SW480, Tram, 72h, rep3 SW480, Tram, 72h,
GSM9340241 SW480, Tram, 8h, rep3 SW480, Tram, 8h,

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GSE312194 MEK inhibition induces AXIN1 loss in colorectal cancer by mTOR associated suppression of protein synthesis [RNASeq_HCT116] 27 samples shares axin1, mek, suppression, inhibition, colorectal, synthesis, induces
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GSE281506 Combining Menin and MEK inhibition to target poor prognosis KMT2A-rearranged RAS pathway-mutant acute myeloid leukemia 42 samples shares mek, inhibition
GSE271504 Effect of BRAF and MEK inhibition in KRAS mutant NSCLC cell line NCI-H358 32 samples shares mek, inhibition
GSE283752 Differential unfolded protein response regulation in KRAS silencing sensitive versus innately resistant colorectal cancer cells [SW480 and SW837] 12 samples shares sw480, colorectal

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