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Transcriptional profile of primary human lung organoid-derived air-liquid interface (ALI) cultures

GSE312700 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/05/05 Platform GPL34284
Summary
Innate defences of the respiratory epithelium are the first barrier against incoming respiratory viruses. To understand the contribution of both basal (tonic) and induced interferon (IFN) to antiviral defences in a physiologically relevant system, we established air-liquid interface (ALI) cultures of primary human bronchial epithelium (HBE) and small airway epithelium (HSE). Via an organoid intermediate stage, the limited healthy donor material was expanded while preserving stemness and subsequently differentiated. We here perform bulk RNA-seq to characterize the transcriptomes and cellular diversity of our ALI cultures.
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Also filed as BioProject PRJNA1375738 and SRA study SRP651783. Searching any of these in the dataset finder brings you back here.

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