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Single-cell RNA-seq of colonic tumors from epithelial HO-1 knockout and control mice in a colitis-associated cancer model

GSE312733 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/12/12 Platform GPL24247
Summary
This dataset contains single-cell RNA sequencing profiles of colonic tumor cells isolated from mice with intestinal epithelial-specific deletion of Hmox1 and littermate controls. Tumors were generated using the azoxymethane-dextran sodium sulfate (AOM-DSS) model of colitis-associated cancer. Following enzymatic dissociation and live cell sorting, single-cell libraries were prepared using the 10x Genomics Chromium platform and sequenced to characterize transcriptional programs within the tumor microenvironment. The dataset was designed to examine genotype-dependent shifts in epithelial transcriptional programs within the tumor microenvironment, with a focus on stress-adaptive responses, iron metabolism, and oxidative stress regulation.
Published in
Epithelial HO-1 regulates iron availability and promotes colonic tumorigenesis in a context-dependent manner
Callahan RC, Curry JC, Bhagavatula G et al. · JCI insight 2026 · PMID 41410530 · doi:10.1172/jci.insight.181032
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Direct links to NCBI, no account and no request form: the whole study as GSE312733_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1375790 and SRA study SRP651824. Searching any of these in the dataset finder brings you back here.

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