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RNA-seq of bronchoalveolar lavage cells from wild-type mice reconstituted with wild-type or Cry1/2 double-knockout bone marrow.

GSE313652 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/07/30 Platform GPL24247
Summary
Chronic obstructive pulmonary disease (COPD) is a progressive inflammatory lung disease characterized by alveolar destruction and impaired tissue repair. Although circadian disruption has been implicated in COPD pathogenesis, the underlying molecular mechanisms remain poorly understood. To examine the hematopoietic contribution of CRY1/2 to lung homeostasis, we generated bone-marrow–chimeric mice by retro-orbitally injecting 3.5 × 10⁶ bone marrow (BM) cells from Cry1/2 double-knockout (dKO) or age- and sex-matched wild-type (WT) donors into lethally irradiated CD45.1 recipient mice. Eight weeks after transplantation, bronchoalveolar lavage (BAL) cells were collected, and RNA-seq was performed to profile transcriptional changes driven by loss of Cry1/2 in the hematopoietic compartment.
Published in
Cryptochrome Loss Drives COPD-like Lung Pathology through Disrupted Alveolar Epithelial Proliferation and Immune Homeostasis
Mills TW, Han C, Lim JY et al. · bioRxiv : the preprint server for biology 2026 · PMID 42239331 · doi:10.64898/2026.05.19.726266
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Direct links to NCBI, no account and no request form: the whole study as GSE313652_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1380468 and SRA study SRP654886. Searching any of these in the dataset finder brings you back here.

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