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Autophagy enhancement ameliorates tau burden and neurofilament pathology in sporadic PSP-RS neurons

GSE313924 Homo sapiens Expression profiling by high throughput sequencing 3 samples Submitted 2026/01/30 Platform GPL34281
Summary
Progressive supranuclear palsy, Richardson’s syndrome subtype (PSP-RS), is a tauopathy marked by early axonal pathology and neurodegeneration. Modeling sporadic PSP-RS in human neurons remained a major challenge. Here, we generated midbrain dopaminergic (mDA) neurons from induced pluripotent stem cells (iPSCs) derived from idiopathic PSP-RS patients and healthy controls. Combined transcriptomic and proteomic analysis revealed reduced dopaminergic differentiation and synaptic function, alongside increased phosphorylated and oligomeric Tau, neurofilament accumulation, axonal swelling, and endoplasmic reticulum disorganization. These alterations coincided with impaired autophagic flux and elevated levels of phosphorylated mTOR. Pharmacological inhibition of mTOR restored autophagy and reduced neurofilament and tau pathology. Collectively, our findings implicate mTOR-dependent autophagy dysfunction as a key driver of early axonal pathology of PSP-RS and highlight autophagy modulation as a promising therapeutic avenue.
Published in
Cytoskeletal Imbalance and Axonal Vulnerability in Sporadic PSP-RS: Early Changes in a Human iPSC-Derived Neuronal Model with Altered mTOR Signaling
Covello R, Benedetto GL, Scalise S et al. · Cells 2026 · PMID 42121856 · doi:10.3390/cells15090754
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Also filed as BioProject PRJNA1387361 and SRA study SRP655577. Searching any of these in the dataset finder brings you back here.

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