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Contact with smooth muscle cells dictates the endothelial transcriptome

GSE314128 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2025/12/31 Platform GPL24676
Summary
The phenotypes of endothelial cells (ECs) in conduit and resistance arteries differ in part due to the presence of myoendothelial junctions in resistance arteries. These are direct contact points between ECs and smooth muscle cells (SMCs) that form through holes in the inner elastic lamina. To investigate the consequences of SCM contact on endothelial phenotypes, we used transwells to culture ECs in three conditions: cultured without SMCs (monolayer ECs), co-cultured without direct contact with SMCs (no-contact ECs), and co-cultured with SMCs such that myoendothelial junctions could form (contact ECs). We then collected the ECs and performed bulk RNA sequencing. These data demonstrate the impact of SMC-derived signals on endothelial phenotypes.
Published in
Arterial iron regulates vasodilation during anemia via endothelial holo α-globin
Dunaway LS, Nyshadham S, Loeb SA et al. · Blood 2026 · PMID 42371808 · doi:10.1182/blood.2025032905
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Also filed as BioProject PRJNA1390136 and SRA study SRP655997. Searching any of these in the dataset finder brings you back here.

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