RNA-sequencing of lineage-traced memory Th2 cells after Heligmosomoidese polygyrus bakeri infection: IL-5 vs. IL-13

Despite being intestine-restricted, the helminth Heligmosomoidese polygyrus bakeri (Hpb) induces systemic accumulation of Th2 cells, providing the opportunity to sequence Th2 cells in multiple tissue of the same mouse. Here we first compared IL-5-Cre and IL-13-Cre for permanent fluorescent labeling of Th2 effector cells after Hpb infection Next, we compared IL-5-Cre traced memory Th2 cells from multiple tissues: mesenteric lymph nodes (MLN), small intestine lamina propria (SILP) and mesenteric fat (MFAT). As a control, we also sorted naive CD4+ T cells from the MLN.