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bulkRNA-seq LSKs CMPs MEPs GMPs from bone marrow and spleen

GSE314281 Mus musculus (mouse) RNA-seq (gene expression) 32 samples Submitted 2026/04/17 Platform GPL17021
At a glance

RNA-seq (gene expression) · mouse · 32 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
32 samples: 8 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Divergent granulopoiesis at extramedullary sites safeguards antibacterial host defenseScience immunology · 2026
Summary (written by the submitters)
Comparison of bone marrow or splenic originated stem cell populations (LSKs, CMPs, MEPs, and GMPs) in a mouse model of emergency granulopoieis.
Published in
Divergent granulopoiesis at extramedullary sites safeguards antibacterial host defense
Silvestre-Roig C, Chevre R, Farjia M et al. · Science immunology 2026 · PMID 41996475 · doi:10.1126/sciimmunol.adw7077
This dataset
Open GSE314281 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE314281_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1390651 and SRA study SRP656379. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41996475) ↗
Study design
8 conditions, mostly with about 4 replicates each
BM_CMP ×4 BM_GMP ×4 BM_LSK ×4 BM_MEP ×4 SP_CMP ×4 SP_GMP ×4 SP_LSK ×4 SP_MEP ×4

Supports a between-group comparison across 32 samples.

How we read this: 8 replicated groups read from 32 sample titles; they account for 32 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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