← BioTransfer GEO Dataset Finder
GEO series

Molecular Indicators of Isometric Exercises Efficacy in Early Rehabilitation of Older Adults after Total Hip Arthroplasty

GSE315228 Homo sapiens (human) RNA-seq (gene expression) 72 samples Submitted 2026/02/18 Platform GPL26167
At a glance

RNA-seq (gene expression) · human · 72 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
72 samples: 24 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Molecular Indicators of Isometric Exercise Efficacy in Early Rehabilitation of Older Adults After Total Hip ArthroplastyInternational journal of molecular sciences · 2026
Summary (written by the submitters)
Developing molecular methods for assessing the effectiveness of physical rehabilitation remains a pressing task. Our study aimed to assess the utility of transcriptome analysis of muscle biopsies in evaluating the efficacy of the isometric exercises (IE) added to the standard protocol in early rehabilitation of older patients during the initial two weeks post total hip arthroplasty (THA).
Read the full summary ↓
Blood concentrations of total leukocytes, neutrophils, IL6, IL8, IL1β, myoglobin, etc. were measured, and transcriptome analysis of rectus femoris biopsies from the operated leg was performed before and after (1 and 12 days) THA in women aged 73–77 years. The additional IE in the experimental rehabilitation group led to a significant acceleration in the recovery of IL6, IL8 and IL1β blood levels to the normal ranges compared to the control group, as confirmed by Fisher’s exact test for this cytokine combination. The results of Gene Set Enrichment Analysis and Differen-tially Expressed Gene Analyses for the muscle biopsies point to accelerated resolution of inflammation, along with enhanced activation of genes associated with angiogenesis, lymphangiogenesis, vasodilation, and vasoconstriction in the experimental rehabilita-tion group compared to the control group. Thus, IL6, IL8 and IL1β blood levels can serve being in combination as molecular indicators of the efficacy of early rehabilitation after THA, and transcriptome analysis of the rectus femoris biopsies of the operated leg allows revealing molecular indicators of regenerative processes in muscle tissue near the surgery area after THA.
Published in
Molecular Indicators of Isometric Exercise Efficacy in Early Rehabilitation of Older Adults After Total Hip Arthroplasty
Maksimova EA, Krasnov KS, Senotov AS et al. · International journal of molecular sciences 2026 · PMID 41683811 · doi:10.3390/ijms27031389
This dataset
Open GSE315228 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE315228_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1395377 and SRA study SRP658818. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41683811) ↗
Study design
24 conditions, mostly in triplicate
rectus femoris, before operation, patient 1, ×3 rectus femoris, 1d after operation, patient 1, ×3 rectus femoris, 12d after operation, patient … ×3 rectus femoris, before operation, patient 2, ×3 rectus femoris, 1d after operation, patient 2, ×3 rectus femoris, before operation, patient 3, ×3 rectus femoris, 1d after operation, patient 3, ×3 rectus femoris, 12d after operation, patient … ×3 +16 more

Supports a between-group comparison across 72 samples.

How we read this: 24 replicated groups read from 72 sample titles; they account for 72 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 32 more — browse all 72 samples with per-sample file links →

Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.