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The phytosterol 24 (S)-saringosterol alters lipid homeostasis and inflammatory pathways in a cell-specific manner.

GSE315287 Mus musculus (mouse) RNA-seq (gene expression) 27 samples Submitted 2026/04/01 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 27 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
27 samples: 9 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
The phytosterol 24(S)-saringosterol alters lipid homeostasis and inflammatory pathways in a cell-specific mannerBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2026
Summary (written by the submitters)
Liver X receptors (LXR alpha/beta) are nuclear receptors that play a crucial role in regulating various biological processes, including lipid metabolism and inflammation. Activation of these receptors using synthetic pan LXR agonists, such as T0901713 and GW3965 has demonstrated neuroprotective effects, making them promising therapeutic targets for neurodegenerative disorders like Alzheimer’s Disease (AD).
Read the full summary ↓
However, the clinical applications of synthetic pan-LXR agonists are limited by adverse hepatic effects including hypertriglyceridemia and steatosis. Thus, dissociating the beneficial effects of LXR agonism from the deleterious side effects in the liver will be required for the development of LXR agonists as better potential therapeutics. In this study, we investigated 24(S)-saringosterol, a phytosterol derived from the seaweed Sargassum fusiforme, which has previously been reported to exhibit LXR agonist activity without inducing hepatic steatosis. To elucidate its effects on lipid metabolism and inflammatory pathways, we used two distinct cell lineages-myeloid and hepatic cells. Our results demonstrate that 24(S)-saringosterol exhibits a stronger activation of LXR regulated gene expressions leading to dose-dependent upregulation of the cholesterol efflux transporter ABCA1 in both cell types. In myeloid cells, including microglia and bone marrow-derived macrophages, like GW3965, 24(S)-saringosterol exhibited anti-inflammatory effects by downregulating genes involved in interferon- pathways and promoted cholesterol efflux by upregulating ABCA1 via LXR activation. In myeloid as well as hepatic cells, 24(S)-saringosterol reduced the expression of cholesterol biosynthesis genes as well as LDLR via SREBP2, in contrast to GW3965, which upregulated expression of lipid synthesis genes via SREBP1. These findings highlight the therapeutic potential of 24(S)-saringosterol as a dissociated LXR agonist that effectively mitigates the adverse effects associated with synthetic LXR agonists. This compound represents a promising candidate for further investigation in the context of AD and other metabolic and inflammatory disorders.
Published in
The phytosterol 24(S)-saringosterol alters lipid homeostasis and inflammatory pathways in a cell-specific manner
Singh M, Yu J, Vanmierlo T et al. · Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2026 · PMID 42107911 · doi:10.1016/j.biopha.2026.119506
This dataset
Open GSE315287 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE315287_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1395582 and SRA study SRP658910. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42107911) ↗
Study design
9 conditions, mostly in triplicate
Bone marrow-derived macrophage treated by GW3… ×3 Hepatocyte treated by GW3596, ×3 Microglia treated by GW3596, ×3 Bone marrow-derived macrophage treated by sar… ×3 Hepatocyte treated by saringosterol, ×3 Microglia treated by saringosterol, ×3 Bone marrow-derived macrophage treated by veh… ×3 Hepatocyte treated by vehicle, ×3 +1 more

Supports a between-group comparison across 27 samples.

How we read this: 9 replicated groups read from 27 sample titles; they account for 27 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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