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Transcriptomic profiling of NNMT knockdown in MKN-45 gastric cancer cells

GSE315562 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/06/08 Platform GPL24676
Summary
MKN-45 gastric cancer cells were subjected to NNMT knockdown or negative control treatment and cultured in three independent biological replicates per group. Total RNA was extracted using TRIzol reagent, and RNA integrity was confirmed with RIN > 8.0. RNA libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit and sequenced on the Illumina NovaSeq 6000 platform with 150 bp paired-end reads. Differential gene expression analysis was performed to identify transcriptomic changes induced by NNMT knockdown.
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Direct links to NCBI, no account and no request form: the whole study as GSE315562_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1398266 and SRA study SRP659722. Searching any of these in the dataset finder brings you back here.

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