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CB001 bioactive compounds improve intestinal immune disorders caused by simulated microgravity and radiation through anti-inflammatory [scRNA-seq]

GSE316111 Mus musculus (mouse) Single-cell RNA-seq 2 samples Submitted 2026/06/10 Platform GPL24247

At a glance

Single-cell RNA-seq · mouse · 2 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism: Mus musculus (mouse)
Samples: 2 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: CB001 bioactive compounds mitigate intestinal immune dysregulation induced by simulated microgravity and radiation via B cells modulationPharmaceutical biology · 2026

Summary (written by the submitters)

Gastrointestinal discomfort is one of the most common issues during spaceflight, and there is currently a lack of targeted drug treatment strategies. Current research in this area focuses on single-factor modeling, lacking a comprehensive understanding. In the present study, we constructed space flight models based on most important two factors, microgravity and low-dose radiation.

Read the full summary ↓

Histological, cellular and molecular analyses confirmed the advantage of this dual-factor model in simulating space flight-caused injuries. Upregulated inflammatory and homeostasis disruption were observed in intestines treated by these dual factors. Our data revealed that dual factors enhanced the migration of a Ccr7+ B cells into intestines through upregulating the expression of Cxcr4, which exerted strong activation regulations on leukocytes. We further validated that bioactive compounds of CB001 effectively alleviated intestinal perturbations through recovering the expression of tight junction proteins, suppressing the migration of B cells and downregulating the levels of pro-inflammatory cytokines. Collectively, our work offered CB001 compounds as candidate intervention drug for space flight-induced intestine dysfunctions based on a dual-factor mice model. These data shed lights on special medicine screening for health protection in space flight.

Published in

CB001 bioactive compounds mitigate intestinal immune dysregulation induced by simulated microgravity and radiation via B cells modulation

Liu J, Bai Z, Dong Y et al. · Pharmaceutical biology 2026 · PMID 42224656 · doi:10.1080/13880209.2026.2675166

This dataset

Open GSE316111 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE316111_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1400621 and SRA study SRP661125. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42224656) ↗

Samples in this study

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+ 2 more — browse all 2 samples with per-sample file links →

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