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Transcriptomic Analysis of HeLa Cells Treated with Enterococcus faecium Supernatant: RNA-seq Reveals Differential Gene Expression

GSE316117 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/05/26 Platform GPL24676
Summary
This study aimed to investigate the transcriptional response of human cervical cancer HeLa cells to metabolites derived from Enterococcus faecium. HeLa cells were treated with filter-sterilized E. faecium supernatant (equivalent to 1×10⁸ CFU/mL) for 24 hours, with three biological replicates each for the control (HC) and treatment (HT) groups. Total RNA was extracted and subjected to transcriptome sequencing. Bioinformatic analysis identified a set of differentially expressed genes involved in cellular metabolism, stress response, and key signaling pathways. The results demonstrate that E. faecium metabolites significantly alter the transcriptional profile of HeLa cells, providing new insights into the molecular mechanisms underlying host–bacterial metabolic interactions.
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Direct links to NCBI, no account and no request form: the whole study as GSE316117_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1400636 and SRA study SRP661145. Searching any of these in the dataset finder brings you back here.

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