GEO series

Human spinal cord organoids recapitulate developmental and disease-associated oligodendrocyte lineage signatures [RNA-Seq]

GSE316395 Homo sapiens (human) RNA-seq (gene expression) 47 samples Submitted 2026/06/01 Platform GPL34284

At a glance

RNA-seq (gene expression) · human · 47 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Samples: 47 samples: 23 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

These data consist of two RNAseq datasets. The first is a timeline of human spinal organoid (hSpO) gene expression from d75 to d200. The second is gene expression of human primary cortical cells which were immunopanned to enrich for oligodendrocytes, then treated with either vehicle, cytokine cocktail of TNF-α, IL-1α, and C1q (TIC), or IFN-gamma.

This dataset

Open GSE316395 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE316395_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1402439. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

23 conditions, mostly in duplicate

hSpO - d75 - Line 1 ×2 hSpO - d75 - Line 2 ×2 hSpO - d75 - Line 3 ×2 hSpO - d100 - Line 1 ×2 hSpO - d100 - Line 2 ×2 hSpO - d100 - Line 3 ×2 hSpO - d120 - Line 1 ×2 hSpO - d120 - Line 2 ×2 +16 more

Supports a between-group comparison across 46 samples.

How we read this: 23 replicated groups read from 47 sample titles; they account for 46 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9451644 hSpO - d75 - Line 1 - 1 hSpO - d75 - Line 1
GSM9451645 hSpO - d75 - Line 1 - 2 hSpO - d75 - Line 1
GSM9451646 hSpO - d75 - Line 2 - 1 hSpO - d75 - Line 2
GSM9451647 hSpO - d75 - Line 2 - 2 hSpO - d75 - Line 2
GSM9451648 hSpO - d75 - Line 3 - 1 hSpO - d75 - Line 3
GSM9451649 hSpO - d75 - Line 3 - 2 hSpO - d75 - Line 3
GSM9451650 hSpO - d100 - Line 1 - 1 hSpO - d100 - Line 1
GSM9451651 hSpO - d100 - Line 1 - 2 hSpO - d100 - Line 1
GSM9451652 hSpO - d100 - Line 2 - 1 hSpO - d100 - Line 2
GSM9451653 hSpO - d100 - Line 2 - 2 hSpO - d100 - Line 2
GSM9451654 hSpO - d100 - Line 3 - 1 hSpO - d100 - Line 3
GSM9451655 hSpO - d100 - Line 3 - 2 hSpO - d100 - Line 3
GSM9451656 hSpO - d120 - Line 1 - 1 hSpO - d120 - Line 1
GSM9451657 hSpO - d120 - Line 1 - 2 hSpO - d120 - Line 1
GSM9451658 hSpO - d120 - Line 2 - 1 hSpO - d120 - Line 2
GSM9451659 hSpO - d120 - Line 2 - 2 hSpO - d120 - Line 2
GSM9451660 hSpO - d120 - Line 3 - 1 hSpO - d120 - Line 3
GSM9451661 hSpO - d120 - Line 3 - 2 hSpO - d120 - Line 3
GSM9451662 hSpO - d150 - Line 1 - 1 hSpO - d150 - Line 1
GSM9451663 hSpO - d150 - Line 1 - 2 hSpO - d150 - Line 1
GSM9451664 hSpO - d150 - Line 2 - 1 hSpO - d150 - Line 2
GSM9451665 hSpO - d150 - Line 2 - 2 hSpO - d150 - Line 2
GSM9451666 hSpO - d150 - Line 3 - 1 hSpO - d150 - Line 3
GSM9451667 hSpO - d150 - Line 3 - 2 hSpO - d150 - Line 3
GSM9451668 hSpO - d200 - Line 1 - 1 hSpO - d200 - Line 1
GSM9451669 hSpO - d200 - Line 1 - 2 hSpO - d200 - Line 1
GSM9451670 hSpO - d200 - Line 2 - 1 hSpO - d200 - Line 2
GSM9451671 hSpO - d200 - Line 2 - 2 hSpO - d200 - Line 2
GSM9451672 hSpO - d200 - Line 3 - 1 hSpO - d200 - Line 3
GSM9451673 hSpO - d200 - Line 3 - 2 hSpO - d200 - Line 3
GSM9451674 Primary - Vehicle - Sample 1 - 1 Primary - Vehicle - Sam…
GSM9451675 Primary - Vehicle - Sample 1 - 2 Primary - Vehicle - Sam…
GSM9451676 Primary - TIC - Sample 1 - 1
GSM9451677 Primary - IFNG - Sample 1 - 1 Primary - IFNG - Sample…
GSM9451678 Primary - IFNG - Sample 1 - 2 Primary - IFNG - Sample…
GSM9451679 Primary - Vehicle - Sample 2 - 1 Primary - Vehicle - Sam…
GSM9451680 Primary - Vehicle - Sample 2 - 2 Primary - Vehicle - Sam…
GSM9451681 Primary - TIC - Sample 2 - 1 Primary - TIC - Sample 2
GSM9451682 Primary - TIC - Sample 2 - 2 Primary - TIC - Sample 2
GSM9451683 Primary - IFNG - Sample 2 - 1 Primary - IFNG - Sample…

+ 7 more — browse all 47 samples with per-sample file links →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.