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Effect of PPARγ2 acetylation in at lysine 382 in mouse beige adipocyte differentiation

GSE317859 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/06/18 Platform GPL34290
Summary
PPARr is a master regulator of adipogenesis which undergo different post translational modification during adipogenesis. This post translational modification of PPARγ control unique subset of genes rather full PPARr target genes. Recently we reported that SIRT7 deacetylate PPARr2 at lysine 382 residue and regulate lipogenesis in C3H10T1/2 cells. Here, we investigate this SIRT7-mediated PPARγ2 lysine deacetylation in mouse beige adipocyte differentiation of subcutaneous white AT (scWAT) stromal vascular fraction (SVF) cell line. At first we prepared PPARr2 K382 acetylation (KQ) and deacetylation (KR) mimicking mutant expressing mouse scWAT SVF cell line. After beige adipocyte differentiation, we isolated total RNA at day 2 and performed RNA-seq analysis.
Published in
SIRT7 Inhibits Adipose Tissue Browning Through Deacetylation of PPARγ2 at K382
Das A, Yoshizawa T, Yamada D et al. · Cells 2026 · PMID 42274620 · doi:10.3390/cells15111028
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Also filed as BioProject PRJNA1415722 and SRA study SRP668839. Searching any of these in the dataset finder brings you back here.

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