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Single-cell RNA sequencing of E18.5 mouse hindstomach epithelium and bulk RNA sequencing of its derived 3D organoids across distinct YAP activity states [scRNA-Seq]

GSE318022 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/05/28 Platform GPL28330
Summary
Hippo signaling plays a crucial role in the development of multiple organs. This study consists of two transcriptomic datasets.Single-cell RNA sequencing was conducted on E18.5 mouse hindstomach epithelial cells of littermate control, YAP5SA-overexpressing and Yap knockout mice, where YAP5SA represents a constitutively active form of Yap. Bulk RNA sequencing was performed to analyze transcriptional changes in 3D organoids derived from the glandular embryonic hindstomach epithelium of control and YAP5SA-overexpressing mice. Among these, organoids were cultured under two conditions: growth medium for 5 days, or growth medium for 5 days followed by differentiation medium for an additional 7 days. Comparative gene expression profiling provides valuable insights into the role of the Hippo signaling pathway in regulating diverse biological processes during glandular stomach epithelial development.
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Direct links to NCBI, no account and no request form: the whole study as GSE318022_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 3 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1416340 and SRA study SRP670447. Searching any of these in the dataset finder brings you back here.

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