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Single cell LARRY state-fate analysis of Npm1cA/Flt3-ITD acute myeloid leukemia stem cells

GSE318348 Mus musculus (mouse) Single-cell RNA-seq 20 samples Submitted 2026/03/01 Platform GPL30172
At a glance

Single-cell RNA-seq · mouse · 20 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism
Mus musculus (mouse)
Samples
20 samples: 20 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
AML leukemia stem cells are principal drivers of leukemia disease. Leveraging novel leukemia stem cell cultures (PLSTCs) we performed state-fate analysis to understand the trajectories of distinct AML LSC clones in vivo and ex vivo using a LARRY expressed barcoding strategy. The results indicate that self-renewing LSCs show clonally-heritable programs that predict in vivo engraftment, fitness and therapy response. Targeting those programs using a CROPseq perturbation and single-cell sequencing approach, we identify a novel mechanism regulating the LSC state.
This dataset
Open GSE318348 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE318348_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1418608 and SRA study SRP675260. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
20 samples, each with a distinct title — no replicated groups

Supports a case/control comparison: 3 samples read as cases, 3 as controls.

How we read this: Read from 20 sample titles: 20 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.