Transcriptomic analysis of colchicine effects in adenine-induced chronic kidney disease

Colchicine is an anti-inflammatory drug that has emerged as a therapeutic option in various inflammatory diseases, but its role in chronic kidney disease (CKD) remains unclear. This study investigates the reno-protective effects of colchicine in an experimental CKD model and its potential role in modulating the NOD-like receptor, pyrin domain containing protein 3 (NLRP3) inflammasome pathway. A CKD animal model was established in C57BL/6 mice by feeding a 2% adenine-containing diet for 4 weeks. Mice received either colchicine (0.3 mg/kg/day) or saline intraperitoneally. RNA sequencing of kidney tissue was performed by E-Biogen Inc. (Seoul, Korea) to capture molecular signatures associated with the therapeutic mechanism of colchicine in CKD, with a focus on inflammatory pathways including nuclear factor-κB and NLRP3 pathways.