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Effect of triazine thiols on steady-state mRNA levels in iPSC-derived hepatocytes

GSE318437 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/03/25 Platform GPL28038
Summary
We previously reported that triazine thiols reduce apolipoprotein B (ApoB) secretion from human iPSC-derived hepatocytes (HLCs) and from humanized mice. To determine whether these compounds affected hepatocyte mRNA levels, we performed bulk RNA sequencing of HLCs treated with the triazine thiol DL-1 or with vehicle (DMSO) for 24 hours. Treatment with triazine thiols only modestly affected steady-state mRNA levels. Of 335 differentially expressed genes, none were directly related to ApoB synthesis, lipoprotein assembly, or secretory pathways. The the most affected mRNAs encoded proteins involved in metallothionein induction, suggesting a metal stress response likely unrelated to the compound’s effects on lipid metabolism. These results imply that DL-1 reduces APO-B secretion through post-transcriptional mechanisms.
Published in
Effect of triazine thiols on steady-state mRNA levels in iPSC-derived hepatocytes
Martinez-Morant C, Liu JT, Jiang YL et al. · microPublication biology 2026 · PMID 41867889 · doi:10.17912/micropub.biology.002062
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Also filed as BioProject PRJNA1418727 and SRA study SRP674627. Searching any of these in the dataset finder brings you back here.

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