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10X Genomics Multiome Assay (GEX + ATAC) of hypothalamic preoptic area in prenatally androgenized and control prepubertal female mice.

GSE318722 Mus musculus (mouse) Single-cell RNA-seq 16 samples Submitted 2026/06/17 Platform GPL24247
At a glance

Single-cell RNA-seq · mouse · 16 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism
Mus musculus (mouse)
Samples
16 samples: 16 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Polycystic ovary syndrome (PCOS) is the most common cause of infertility in women. The etiology of PCOS is not well understood. Both genetics and early life exposures are considered contributing factors. Women with PCOS have elevated androgens, potentially exposing female offspring to high androgens in utero. Daughters of women with PCOS are at increased risk of developing this disorder.
Read the full summary ↓
Prenatal androgenization (PNA) of several species, including mice, recapitulates many phenotypes observed in PCOS. Adult female PNA mice exhibit elevated luteinizing hormone (LH) levels and increased gonadotropin-releasing hormone (GnRH) neuron pulse frequency. These changes in GnRH neurons emerge during the pubertal transition. We utilized the 10X Genomics Multiome (gene expression and ATAC-seq) assay to investigate changes in both gene expression and chromatin accessibility in the hypothalamic preoptic area of prepubertal (postnatal day 21) control and PNA females. Through analysis of ~51,000 cells across 34 cell populations, we identified cell-type-specific chromatin remodeling in response to prenatal androgen exposure, despite the absence of coordinated transcriptional changes at postnatal day 21, suggesting that critical developmental programming occurs earlier with persistent epigenetic consequences.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE318722_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1419840 and SRA study SRP675282. Searching any of these in the dataset finder brings you back here.

Study design
16 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 16 sample titles: 16 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.