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RNA-seq analysis of Tada2b knockout in B16F10 cells

GSE318799 Mus musculus Expression profiling by high throughput sequencing 9 samples 2026/04/29 GPL24247
Summary
Tada2b (Transcriptional adaptor 2B) is a core component of the SAGA complex, where it serves as a critical adaptor for the histone acetyltransferase Gcn5. While Tada2b is known to facilitate gene activation, its specific role in melanoma and the maintenance of the transcriptome remains poorly defined. In this study, we investigated the global gene expression changes resulting from Tada2b loss in B16F10 murine melanoma cells. We generated Tada2b-knockout (KO) cells using CRISPR/Cas9 and performed bulk RNA-sequencing. Our goal was to identify differentially expressed genes and dysregulated pathways that depend on Tada2b-mediated transcriptional regulation.
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