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Effect of HIF-1alpha and HIF-2alpha on hypoxic response in Panc1 cells

GSE319526 Homo sapiens (human) RNA-seq (gene expression) 36 samples Submitted 2026/02/18 Platform GPL15433
At a glance

RNA-seq (gene expression) · human · 36 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
36 samples: 36 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
The HIF-2 transcription factor mediates resistance to ferroptosis in pancreatic cancerMolecular cell · 2026
Summary (written by the submitters)
The hypoxic response is influence by both the tissue of origin and local environmental influences, and mediated in large part by the hypoxia-inducible transcription factors HIF-1 and HIF-2. We looked at Panc1 cells, a pancreatic adenocarcinoma cell line, and compared the response to hypoxia in both regular tissue culture media and in pancreatic TIFM (tumor interstitial fluid media), and assessed the degree to which HIF-1 and HIF-2 knockout mediates changes in the hypoxia response.
Published in
The HIF-2 transcription factor mediates resistance to ferroptosis in pancreatic cancer
Hubbi ME, Wang CL, Suhail Y et al. · Molecular cell 2026 · PMID 41932308 · doi:10.1016/j.molcel.2026.03.007
This dataset
Open GSE319526 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE319526_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1423676 and SRA study SRP677073. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41932308) ↗
Study design
36 samples, each with a distinct title — no replicated groups

Supports a case/control comparison: 27 samples read as cases, 9 as controls.

How we read this: Read from 36 sample titles: 36 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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