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Transcriptional regulation by TES and TEAD1 in SNB19 glioblastoma cells: Cut&Tag

GSE319889 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2026/02/23 Platform GPL24676
Summary
This study uses Cleavage Under Targets and Tagmentation (Cut&Tag) chromatin profiling to map genome-wide binding sites of TES (TEAD1 Epigenetic Silencer, an engineered epigenetic silencer factor consisting of KRAB-hTEAD1(1-166)-DNMT3A/3L with V5 tag) and overexpressed wild-type TEAD1 (HA-tagged) in SNB19 glioblastoma cells. Cut&Tag was performed using the CUTANA protocol (EpiCypher). Three biological replicates were prepared for TES (detected via anti-V5) and TEAD1 (detected via anti-HA), with mouse IgG and rabbit IgG as negative controls. Peak calling identified binding sites for both factors, showing that TES largely preserves the DNA-binding specificity of wild-type TEAD1 while converting it into a transcriptional repressor.
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Direct links to NCBI, no account and no request form: the whole study as GSE319889_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1425481 and SRA study SRP678017. Searching any of these in the dataset finder brings you back here.

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