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RSPO2 synergizes with BMP2 to enhance wnt-dependent osteoblast differentiation

GSE320324 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/07/17 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × Control vs 3 × RSPO2 vs 3 × BMP2 vs 3 × BMP2&RSPO2Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Canonical Wnt signaling plays a critical role in osteoblast differentiation and bone formation and is enhanced by R-spondin (RSPO) proteins. Bone morphogenetic protein-2 (BMP2) is a clinically used osteogenic factor whose regenerative efficacy is limited by dose-dependent adverse effects. We recently identified RSPO2 as a contextual enhancer of BMP2-induced bone formation, but the transcriptional mechanisms underlying this synergy remain incompletely understood.
Read the full summary ↓
To define the molecular basis of BMP2–RSPO2 cooperation, we performed RNA sequencing (RNA-seq) analysis of MC3T3-E1 osteoblast precursor cells treated with vehicle control, RSPO2, BMP2, or BMP2+RSPO2 for 5 days under osteogenic conditions. Transcriptomic analysis revealed distinct and overlapping gene expression programs regulated by BMP2 and RSPO2, with co-treatment inducing a unique transcriptional signature enriched for genes associated with osteoblast differentiation, Wnt signaling, and cytoskeletal regulation. These data provide mechanistic insight into how RSPO2 potentiates BMP2-induced osteogenesis and support a role for RSPO2 as a therapeutic co-factor to enhance BMP2-mediated bone regeneration.
This dataset
Open GSE320324 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE320324_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1427598 and SRA study SRP678985. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗
Study design
3 × Control vs 3 × RSPO2 vs 3 × BMP2 vs 3 × BMP2&RSPO2

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.