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Short-Duration HIPEC-Mimetic Exposure to Mithramycin A Induces Structured Transcriptional Reprogramming in HT-29 Colorectal Cancer Cells

GSE320366 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2026/05/15 Platform GPL24676
Summary
This study investigates the transcriptional consequences of short-duration HIPEC-mimetic exposure to Mithramycin A (MA) in HT-29 colorectal cancer cells. Cells were exposed for 90 minutes to MA (750 nM) or Mitomycin C (4.48 µM) at 37 °C followed by drug washout. RNA sequencing was performed to determine whether transient exposure induces durable transcriptional reprogramming. Differential expression and pathway analyses identified coordinated enrichment of cell-cycle checkpoint programs and modulation of chromatin regulatory networks. One biological replicate (MA2) was excluded due to high duplication rates and low mapping efficiency. Analyses excluding this sample are presented.
Published in
Short-Duration HIPEC-Mimetic Mithramycin A Exposure Induces Durable Transcriptional Remodeling Involving Chromatin Regulatory Networks in Colorectal Cancer Models
Coburn-Flynn O, Butchy MV, Ghanem Y et al. · International journal of molecular sciences 2026 · PMID 42074218 · doi:10.3390/ijms27083580
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Also filed as BioProject PRJNA1427673 and SRA study SRP679068. Searching any of these in the dataset finder brings you back here.

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