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A novel nanobody-based TCR-like CAR-T therapy targeting PRAME for the treatment of acute myeloid leukemia

GSE320438 Homo sapiens (human) RNA-seq (gene expression) 12 samples Submitted 2026/04/25 Platform GPL34284
At a glance

RNA-seq (gene expression) · human · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Topic
Acute myeloid leukemiaThis series is part of our curated briefing on it.
Samples
12 samples: 3 × untransduced T cells vs 3 × anti-PRAME CAR-T cells vs 3 × anti-PRAME CAR-T cells cocultured with THP-1 vs 3 × anti-PRAME CAR-T cells cocultured with OCI-AML3Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
A Novel Nanobody-Based TCR-like CAR T Therapy Targeting PRAME for the Treatment of Acute Myeloid LeukemiaClinical cancer research : an official journal of the American Association for Cancer Research · 2026
Summary (written by the submitters)
Chimeric antigen receptor (CAR) T-cell immunotherapy in acute myeloid leukemia (AML) remains challenging, primarily due to the lack of specific cell surface antigens that are highly expressed on leukemic blasts but largely absent on hematopoietic stem/progenitor cells and healthy tissues. TCR-like CAR-T therapy targeting intracellular antigens provides a novel strategy for the treatment of AML.
Read the full summary ↓
In this study, we aimed to develop nanobodies targeting the intracellular antigen PRAME and to establish a novel nanobody-based TCR-like CAR-T cell therapy directed against the peptide-MHC complex PRAME425-433/HLA-A2.
Published in
A Novel Nanobody-Based TCR-like CAR T Therapy Targeting PRAME for the Treatment of Acute Myeloid Leukemia
Liu X, Li J, Li X et al. · Clinical cancer research : an official journal of the American Association for Cancer Research 2026 · PMID 41985065 · doi:10.1158/1078-0432.CCR-25-2726
This dataset
Open GSE320438 in finder → Find more human RNA-seq datasets → Acute myeloid leukemia landscape →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE320438_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1428207 and SRA study SRP679270. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41985065) ↗
Study design
3 × untransduced T cells vs 3 × anti-PRAME CAR-T cells vs 3 × anti-PRAME CAR-T cells cocultured with THP-1 vs 3 × anti-PRAME CAR-T cells cocultured with OCI-AML3

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
More Acute myeloid leukemia datasets

Search all human RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.