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Langerhans cells regulate immunity in adulthood by regulating dermal lymphatic development in early life

GSE322556 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/06/19 Platform GPL34290
Summary
The communication between skin and draining lymph nodes is crucial for immune responses to skin insults. Lymphatic vessels play critical roles in this communication by transporting antigens from skin to lymph nodes, and mechanisms that compromise dermal lymphatic function can disrupt immunity. Here, we show that Langerhans cells (LCs), epidermis-derived antigen-presenting cells, have a lymphatic-regulating role in promoting dermal lymphatic expansion and phenotype acquisition during early life. This function is mediated by PlGF and VEGF-C, and LCs have a large impact uniquely on the transcriptomic profile of lymphatic endothelial cells compared to other skin cells. Compromise of this early life LC-mediated lymphatic expansion results in reduced soluble antigen flow to draining lymph nodes and T cell responses in adulthood. Our data provide a tissue-based mechanism by which LCs regulate T cells remotely across time and space and raise the possibility that immune diseases in adulthood could reflect compromise of the LC-lymphatic axis in childhood.
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Direct links to NCBI, no account and no request form: the whole study as GSE322556_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 3 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1430420 and SRA study SRP680226. Searching any of these in the dataset finder brings you back here.

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