Single Cell Transcriptomic Analysis Demonstrating Oxygen Dependence of Human Hematopoietic Stem and Progenitor Cells

Hematopoietic stem and progenitor cells support a lifetime supply of blood and immune cells, can become neoplastic when dysregulated, and constitute a powerful cell therapy vehicle for hematologic diseases. We demonstrated that human hematopoietic cell numbers, growth, biochemical properties, and functional potency is affected by variation in physiologically and clinically relevant local oxygen tensions. Lineage defined progenitor cells showed increased expansion in high oxygen, while primitive cells and those with in vivo potency were maintained at higher frequencies in low physiologic oxygen. Here we present a novel hematopoietic cell oxygen-dependent single cell transcriptomic profile to determine changes in gene expression induced by exposure to various physiologically relevent oxygen tensions.