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Cross-Species Transcriptomic Integration Reveals a Conserved, MIRO1-Mediated Macrophage-to-T Cell Signaling Axis Driving Immunosuppression in Glioma [RNA-Seq]

GSE322979 Homo sapiens Expression profiling by high throughput sequencing 7 samples Submitted 2026/03/05 Platform GPL34284
Summary
We generated bulk RNA sequencing (RNA-seq) data from human glioma surgical resections treated ex vivo with a MIRO1-binding compound (MR3) or vehicle control. Fresh tumor specimens were processed and cultured under controlled conditions prior to RNA extraction and high-throughput transcriptomic profiling to characterize treatment-associated gene expression changes within the tumor microenvironment (TME). The dataset captures global transcriptional programs from tumor tissue, reflecting contributions from malignant cells as well as stromal and immune components present in the resections. These data enable analysis of MIRO1-dependent transcriptional regulation in human glioma and facilitate cross-species integration with murine single-nucleus RNA-seq datasets. This resource supports investigation of mitochondrial-associated transcriptional programs and their impact on immune microenvironment remodeling in human glioma.
Published in
Cross-Species Transcriptomic Integration Reveals a Conserved, MIRO1-Mediated Macrophage-to-T Cell Signaling Axis Driving Immunosuppression in Glioma
Du Z, Li M, Bergsneider BH et al. · bioRxiv : the preprint server for biology 2025 · PMID 41292883 · doi:10.1101/2025.11.10.686781
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Direct links to NCBI, no account and no request form: the whole study as GSE322979_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1432038 and SRA study SRP680970. Searching any of these in the dataset finder brings you back here.

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