← BioTransfer GEO Dataset Finder
GEO series

SEPHS2 deficiency reprograms glucose metabolism to activate the pentose phosphate pathway by stabilizing PCK1 through NAD+

GSE324223 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/03/11 Platform GPL24676
Summary
SEPHS2, a key enzyme in synthesizing selenoproteins, which converts hydrogen selenide into selenophosphate. We found that SEPHS2 knockout inhibits oxidative phosphorylation (OXPHOS), but has no effect on glycolysis, as indicated by an seahorse assay. SEPHS2 loss redirects more glucose to the pentose phosphate pathway (PPP). Mechanistically, SEPHS2 knockout elevates NAD+ levels, which are essential for Sirtuin activity. The increase in the activity of SIRT2 leads to deacetylation of PCK1, a posttranslational modification required to prevent PCK1’s ubiquitination and subsequent degradation. Thus, SEPHS2 knockout stabilizes PCK1 by reducing its ubiquitination. Since PCK1 is a key regulator of gluconeogenesis, SEPHS2 knockout promotes gluconeogenesis while reducing cellularglucose uptake, down-regulating oxidative phosphorylation and enhancing the PPP.
Published in
SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization
Zhang Y, Zhang Q, Wei B et al. · Cell reports 2026 · PMID 42035418 · doi:10.1016/j.celrep.2026.117297
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE324223_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1334283. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.