GEO series

Distribution of big tau isoforms in the human central and peripheral nervous system

GSE324361 Homo sapiens (human) RNA-seq (gene expression) 10 samples Submitted 2026/03/10 Platform GPL28352

At a glance

RNA-seq (gene expression) · human · 10 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Samples: 10 samples: 10 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

This project aims to characterize the distribution of “big tau,” a longer tau isoform expressed in the peripheral nervous system (PNS) and select central nervous system (CNS) regions, and to examine its relationship with aging and neurodegeneration. We performed mass spectrometric sequencing of big tau sequence and mapped its distribution across the human nervous system.

Read the full summary ↓

Postmortem samples included brains from Alzheimer’s disease (AD), disease controls, and amyotrophic lateral sclerosis (ALS); spinal cord from young controls, disease controls and ALS; and peripheral nerves. Big and small tau levels were also quantified in the cerebrospinal fluid (CSF) from young normal controls, amyloid positive and amyloid negative participants. To reinforce the presence of big tau in the brain, iso-seq was performed on post-mortem brain tissue samples.

This dataset

Open GSE324361 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE324361_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1434476 and SRA study SRP682355. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

10 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 10 sample titles: 10 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9574411 UWA_6904 WT Caudate CAU_1
GSM9574412 NBB_2020-054 WT Caudate CAU_2
GSM9574413 HBCC_1987 WT Caudate CAU_3
GSM9574414 HBCC_2871 WT Caudate CAU_4
GSM9574415 NBB_2019-116 WT Caudate CAU_5
GSM9574416 BSHRI_2015-1433 WT Middle Frontal Gyrus MFG_6
GSM9574417 BSHRI_2015-280 WT Middle Frontal Gyrus MFG_7
GSM9574418 BSHRI_2015-735 WT Middle Frontal Gyrus MFG_8
GSM9574419 BSHRI_2015-1431 WT Middle Frontal Gyrus MFG_9
GSM9574420 BSHRI_2015-1401 WT Middle Frontal Gyrus MFG_10

Datasets on a similar topic

GSE280720 Functional characterization of Central Nervous System (CNS) and peripheral immune responses in Alzheimer's Disease after Bacillus Calmette Guerin (BCG) vaccination in older adults. 64 samples shares peripheral, central, nervous, system
GSE248094 Systematic characterization of single-cell full-length RNA isoforms in human colorectal cancer 51 samples shares isoforms, system
GSE305125 Molecular Signatures of Peripheral and Central Dental Pulp Subpopulations in Response to Streptococcus mutans: A Bulk RNA-Seq Analysis 36 samples shares peripheral, central
GSE254176 Dynamic changes of immune cells in systemic lupus erythematosus with nephritis using longitudinal peripheral blood single-cell RNA-seq 57 samples shares peripheral, system
GSE327707 Sorted bulk RNA-seq profiling of human peripheral blood mononuclear cell populations across diverse in vitro immune stimuli 150 samples shares peripheral
GSE202216 Rhinovirus-induced gene expression patterns that distinguish between neutrophilic asthma and other asthma inflammatory phenotypes in human peripheral blood mononuclear cells 102 samples shares peripheral
GSE281106 Single-cell RNA-seq of peripheral blood links cell-type-specific regulation of splicing to autoimmune and inflammatory diseases [scRNAseq_KR_SGI] 352 samples shares peripheral
GSE295224 Dual single-cell and bulk RNA sequencing reveal transcriptional profiles underlying heterogenous host-parasite interactions in human peripheral blood mononuclear cells 42 samples shares peripheral

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.