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Role of HIV-1 in IL-8 response of human stellate cells to gram-positive microbial products

GSE324653 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 11 samples Submitted 2026/03/13 Platform GPL34281
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 11 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Homo sapiens (human)
Samples
11 samples: 3 × Lx2 cells, PBS treatment for 24hrs,Repeat vs 3 × Lx2 cells, HIV-1-Bal infetion for 24hrs,Repeat vs 3 × Lx2 cells, HIV-1-Bal infetion with LTA stimulation for 24hrs,RepeatGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Role of HIV-1 in IL-8 response of human stellate cells to gram-positive microbial productsScientific reports · 2026
Summary (written by the submitters)
Patients living with Human Immunodeficiency Virus (PLWH) develop accelerated liver fibrosis, but the exact mechanism remains unknown. Unlike gram-negative bacterial products, the impact of gram-positive microbial products in Human Immunodeficiency Virus 1 (HIV-1) associated liver inflammation and fibrosis remains poorly understood. In this study, we investigated the effect of lipoteichoic acid (LTA), a major gram-positive bacterial component, on HSCs in the context of HIV-1 infection.
Read the full summary ↓
Our data revealed that HIV-1 infection sensitizes HSCs to LTA stimulation, leading to an amplified IL-8 response mediated through histone acetylation that may accelerate liver fibrosis progression in PLWH. As microbial translocation persists despite effective antiretroviral therapy, these findings underscore the need for targeted strategies to mitigate liver fibrosis in HIV-1 infected patients.
Published in
Role of HIV-1 in IL-8 response of human stellate cells to gram-positive microbial products
Ali RO, Zhang L, Chamroonkul N et al. · Scientific reports 2026 · PMID 42297971 · doi:10.1038/s41598-026-56186-1
This dataset
Open GSE324653 in finder → Find more human ChIP / ATAC / CUT&Tag datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE324653_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1436502 and SRA study SRP683160. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42297971) ↗
Study design
3 × Lx2 cells, PBS treatment for 24hrs,Repeat vs 3 × Lx2 cells, HIV-1-Bal infetion for 24hrs,Repeat vs 3 × Lx2 cells, HIV-1-Bal infetion with LTA stimulation for 24hrs,Repeat

Supports a between-group comparison across 9 samples.

How we read this: 3 replicated groups read from 11 sample titles; they account for 9 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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