Single-nucleus transcriptomic profiling of mouse hearts following angiotensin II-infused with or without bisphenol F (BPF) treatment.

Bisphenol F (BPF) is a widely used substitute for bisphenol A (BPA) in plastic manufacturing; however, its potential toxicity remains insufficiently characterized. In this study, we investigated the impact of BPF exposure on the intestinal barrier and cardiovascular system. Based on prior untargeted metabolomic analysis, we revealed that BPF can be converted into N-acetylputrescine (NAP) through a microbiota-associated metabolic process. Further experiments demonstrated that BPF exposure stimulated intestinal epithelial cells to secrete spermidine/spermine N1-acetyltransferase 1 (Sat1), an enzyme involved in this conversion. To identify the cell types and signaling pathways associated with BPF exposure under hypertensive conditions, we conducted snRNA-seq of heart tissues from angiotensin II-infused mice and angiotensin II-infused mice pretreated with BPF exposure. The raw sequencing data and processed expression matrices are provided in this submission.