← BioTransfer GEO Dataset Finder
GEO series

Establishment and Characterization of a Novel Murine Hepatoma Cell Line Allowing Concomitant Conditional Inactivation of Caspase-8 and IKKγ/NEMO

GSE325078 Mus musculus (mouse) RNA-seq (gene expression) 1 samples Submitted 2026/04/01 Platform GPL18480
At a glance

RNA-seq (gene expression) · mouse · 1 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
1 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Caspase-8 and the NF-kappa-B essential modulator (NEMO, also referred to as IKKγ) play critical roles in controlling TNF-α-induced cell death and survival in hepatocytes. The aim of this study was to generate a hepatocyte-derived cell line in which the Casp8 and Nemo genes can be conditionally inactivated simultaneously to investigate the significance of the corresponding signaling pathways.
Read the full summary ↓
To this end, we induced hepatocellular carcinoma in Casp8f/fNemof/f mice using diethylnitrosamine (DEN) and established an immortalized hepatoma cell line from explanted liver tumors, which is subsequently referred to as ΔCN60. ΔCN60 cells still retain floxed Casp8 and Nemo alleles, allowing for efficient Cre-mediated deletion to generate Casp8ΔNemoΔ derivatives. Loss of both Caspase-8 and NEMO inhibits cell proliferation, increases expression of tumor and progenitor markers (AFP, CD133), reduces albumin expression, and blocks TNF-α-induced NF-κB p65 nuclear translocation. Casp8ΔNemoΔ ΔCN60 cells show increased RIP1 stability, altered RIP3 dynamics, and heightened sensitivity to prolonged TNF-α exposure, suggesting a shift towards necroptotic signaling. ΔCN60 serves as a versatile hepatoma model for investigating Caspase-8/NEMO/RIP1/RIP3-dependent pathways and TNF-α-induced liver damage, reducing the need for animal testing.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE325078_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1438597 and SRA study SRP684408. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 1 more — browse all 1 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.