GEO series

Type II IL-4 receptor complex drives mesenchymal fibroblast activation in keloids via STAT6 signaling

GSE325137 Homo sapiens (human) Single-cell RNA-seq 13 samples Submitted 2026/10/01 Platform GPL24676

At a glance

Single-cell RNA-seq · human · 13 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism: Homo sapiens (human)
Samples: 13 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Keloids result from aberrant wound healing characterized by excessive collagen and mesenchymal-related protein production. The type II IL-4 receptor complex, composed of IL-4Rα and IL-13Rα1, is implicated in keloid pathogenesis but remains underexplored. To address this, we applied single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) to healthy normal skin, adjacent normal skin, active keloids, and regressed keloids.

Read the full summary ↓

Integrated scRNA-seq and ST analyses identified IL4R+/IL13RA1+/POSTN+ fibroblasts with combined reticular and mesenchymal phenotypes that were enriched in fibrotic regions of keloids. Immunofluorescence studies confirmed co-localization of IL4R, IL13RA1, and POSTN proteins in keloid tissue. Elevated mRNA levels of IL4R, IL13RA1, COL1A1, FN1, and POSTN were found in both keloid tissue and fibroblasts compared to normal skin. Furthermore, silencing IL13RA1 or IL4R using siRNA in keloid fibroblasts (KFs) resulted in reduced expression of COL1A1, FN1, and POSTN. Lastly, inhibiting type II receptor signaling with dupilumab, an anti-IL4R monoclonal antibody, suppressed IL-13-induced COL1A1, FN1, and POSTN protein expression in KF and collagen deposition in keloid explants. In conclusion, our in vitro and ex vivo findings suggest that inhibition of type II IL-4 receptor signaling with dupilumab may reduce mesenchymal protein expression and collagen deposition, warranting further investigation of this pathway in keloid disease.

This dataset

Open GSE325137 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE325137_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1438695. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 13 more — browse all 13 samples with per-sample file links →

Datasets on a similar topic

GSE285246 Spatially resolved atlas of IPF fibroblastic foci identifies that PAK2 kinase activation is involved in fibrogenesis 12 samples shares fibroblast, activation
GSE273882 Arginine Promotes The Activation of Human Lung Fibroblasts Independent of Its Metabolism 52 samples shares fibroblast, activation
GSE251807 Investigation of gene expression changes in normal human dermal fibroblasts after exposure to bone marrow mesenchymal stromal cell secretome 32 samples shares mesenchymal, fibroblast
GSE267788 Mechanical stretch induces senescence of alveolar epithelial cells and drives fibroblast activation by paracrine mechanisms 25 samples shares fibroblast, activation
GSE264550 Machine learning identifies activation of RUNX/AP-1 as drivers of mesenchymal and fibrotic regulatory programs in gastric cancer 25 samples shares mesenchymal, activation
GSE216842 Transcriptomic landscape of human primary stromal cells (fibroblasts, endothelial cells and mesenchymal stem cells) upon therapy-induced senescence (genotoxic chemotherapy） 24 samples shares mesenchymal, fibroblast
GSE298023 Location and etiology modulate fibroblast activation in the failing human heart 18 samples shares fibroblast, activation
GSE289881 Exploring fibroblast activation protein as an early biomarker in chronic lung allograft dysfunction 10 samples shares fibroblast, activation

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.