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Dendritic cell redundancy enables priming of anti-tumor CD4 T cells in pancreatic cancer

GSE325195 Mus musculus (mouse) RNA-seq (gene expression) 30 samples Submitted 2026/03/18 Platform GPL24247Platform GPL21273
At a glance

RNA-seq (gene expression) · mouse · 30 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
30 samples: 8 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Dendritic cell redundancy enables priming of anti-tumor CD4(+) T cells in pancreatic cancerCancer cell · 2026
Summary (written by the submitters)
Pancreatic ductal adenocarcinoma (PDAC) is resistant to current immunotherapies and lacks effective CD8 T cell responses, which is potentially due to a paucity of cross-presenting dendritic cells (cDC1s). Here, we combine an innate immune adjuvant with anti-CTLA-4 and anti-PD-1 to achieve durable remissions and immunologic memory in multiple mouse models of poorly immunogenic PDAC.
Read the full summary ↓
Tumor regression does not depend on CD8 T cells or tumor cell MHC class I expression but instead requires IFNγ-producing CD4 T cells (Th1s) that were primed by dendritic cells in lymph nodes. Combination immunotherapy induces an influx of activated cDC2s carrying tumor antigen into tumor-draining lymph nodes; cDC2s are required for orthotopic tumor clearance. Intratumoral CD4 T cells and cDC2s remain present in treatment-naïve and chemotherapy-exposed human PDAC. Even in chemotherapy-exposed patients’ blood, cDC2s outnumber cDC1s by ten-fold. Therefore, therapeutic targeting of the cDC2-CD4 T cell axis could be efficacious in pancreatic cancer.
Published in
Dendritic cell redundancy enables priming of anti-tumor CD4(+) T cells in pancreatic cancer
Kureshi CTS, Walsh MJ, Kureshi R et al. · Cancer cell 2026 · PMID 42102812 · doi:10.1016/j.ccell.2026.04.005
This dataset
Open GSE325195 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE325195_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1438987 and SRA study SRP684712. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42102812) ↗
Study design
8 conditions, mostly in triplicate
B16-vehicle ×3 B16-IFNy ×3 B16-IFNb ×3 B16-STING ×3 C2-vehicle ×3 C2-IFNy ×3 C2-IFNb ×3 C2-STING ×3 +6 more

Supports a case/control comparison: 2 samples read as cases, 6 as controls.

How we read this: 8 replicated groups read from 30 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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