← BioTransfer GEO Dataset Finder
GEO series

HP1B and H3K9me3 Regulate Olfactory Receptor Choice and 2 Transcriptional Identity [RNA-Seq]

GSE325634 Mus musculus (mouse) RNA-seq (gene expression) 24 samples Submitted 2026/04/22 Platform GPL21626
At a glance

RNA-seq (gene expression) · mouse · 24 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
24 samples: 8 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
HP1β and H3K9me3 Regulate Olfactory Receptor Choice and Transcriptional IdentityInternational journal of molecular sciences · 2026
Summary (written by the submitters)
Diverse epigenetic regulatory mechanisms ensure and regulate cellular diversity. Among others, the histone 3 lysine 9 me3 (H3K9me3) post translational modification participates in silencing lineage-inappropriate genes. H3K9me3 restricts access of transcription factors and other regulatory proteins to genes that control cell fate. Mouse olfactory sensory neurons (OSN) select to express one olfactory receptor (OR) gene out of 2,600 possibilities.
Read the full summary ↓
This monoallelic and stochastic OR choice happens as OSNs differentiate and undergo dramatic changes in nuclear architecture. OR genes from different chromosomes converge into specialized nuclear bodies and chromatin compartments, as H3K9me3 and chromatin binding proteins including heterochromatin protein 1 (HP1) are incorporated. In this work, we have uncovered an unexpected role for HP1b in OR choice and neuronal identity that cannot be rescued by HP1a in vivo. With the use of a conditional knock-in mouse model that replaces HP1b for HP1a, we observe changes in H3K9me3 levels and DNA accessibility over OR gene clusters. These changes alter the expression paTerns that partition the mouse olfactory epithelium into five OR expression zones, which results in a reduced OR repertoire leading to a loss of olfactory sensory neuron diversity. We propose that HP1b modulates the competition of OR-promoters for enhancers to promote receptor diversity, by establishing repression gradients in a zonal fashion.
Published in
HP1β and H3K9me3 Regulate Olfactory Receptor Choice and Transcriptional Identity
Escamilla-Del-Arenal M, Duffié R, Shayya H et al. · International journal of molecular sciences 2026 · PMID 41977146 · doi:10.3390/ijms27072958
This dataset
Open GSE325634 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE325634_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1441390 and SRA study SRP685857. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41977146) ↗
Study design
8 conditions, mostly in triplicate
Tissue zone 1, Cbx(ctrl) mouse ×3 Tissue zone 5, Cbx(ctrl) mouse ×3 Tissue zone 1, CbxSwap mouse ×3 Tissue zone 5, CbxSwap mouse ×3 mOSN, Cbx(ctrl) mouse ×3 mOSN, CbxSwap mouse ×3 Embryo 17.5, Tmla(ctrl) mouse ×3 Embryo 17.5, Tmla mutant for HP1B ×3

Supports a case/control comparison: 3 samples read as cases, 12 as controls.

How we read this: 8 replicated groups read from 24 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.