Bulk RNA Sequencing of Chimera-Derived Monocyte-Derived Macrophages Targeted for Mitochondrial ROS During Peak Experimental Autoimmune Encephalomyelitis
Single-cell RNA-seq · mouse · 14 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Mus musculus (mouse)
- Samples
- 14 samples: 7 × Sorted mCAT MdMs CD45.2 CCR2creERT2/+ mcat lsl/wt dono vs 6 × Sorted wt MdMs CD45.1 donoGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Monocyte-derived macrophages drive neurological tissue damage through mitochondrial reactive oxygen speciesScience immunology · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE326335_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1434700 and SRA study SRP682495. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 13 samples.
How we read this: 2 replicated groups read from 14 sample titles; they account for 13 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM9629555 Sorted wt MdMs CD45.1 donor
- GSM9629556 Sorted wt MdMs CD45.1 donor_2 Sorted wt MdMs CD45.1 d…
- GSM9629557 Sorted mCAT MdMs CD45.2 CCR2creERT2/+ mcat lsl/wt donor_1 Sorted mCAT MdMs CD45.2…
- GSM9629558 Sorted wt MdMs CD45.1 donor_3 Sorted wt MdMs CD45.1 d…
- GSM9629559 Sorted mCAT MdMs CD45.2 CCR2creERT2/+ mcat lsl/wt donor_2 Sorted mCAT MdMs CD45.2…
- GSM9629560 Sorted mCAT MdMs CD45.2 CCR2creERT2/+ mcat lsl/wt donor_3 Sorted mCAT MdMs CD45.2…
- GSM9629561 Sorted wt MdMs CD45.1 donor_4 Sorted wt MdMs CD45.1 d…
- GSM9629562 Sorted mCAT MdMs CD45.2 CCR2creERT2/+ mcat lsl/wt donor_4 Sorted mCAT MdMs CD45.2…
- GSM9629563 Sorted wt MdMs CD45.1 donor_5 Sorted wt MdMs CD45.1 d…
- GSM9629564 Sorted mCAT MdMs CD45.2 CCR2creERT2/+ mcat lsl/wt donor_5 Sorted mCAT MdMs CD45.2…
- GSM9629565 Sorted wt MdMs CD45.1 donor_6 Sorted wt MdMs CD45.1 d…
- GSM9629566 Sorted mCAT MdMs CD45.2 CCR2creERT2/+ mcat lsl/wt donor_6 Sorted mCAT MdMs CD45.2…
- GSM9629567 Sorted wt MdMs CD45.1 donor_7 Sorted wt MdMs CD45.1 d…
- GSM9629568 Sorted mCAT MdMs CD45.2 CCR2creERT2/+ mcat lsl/wt donor_7 Sorted mCAT MdMs CD45.2…
- GSE287323 Transcriptomic profiling of oligodendrocytes, astrocytes, and microglia in an experimental autoimmune encephalomyelitis (EAE) model of demyelination 19 samples shares encephalomyelitis, experimental, autoimmune
- GSE335147 Mitochondrial profiling across macrophage states reveals inhibition of IL-4/IL-13 reprogramming by the integrated stress response 24 samples shares ros, mitochondrial
- GSE250056 Targeting chemokine crosstalk between T-cells and macrophages to treat immune checkpoint inhibito-mediated myocarditis 68 samples shares ros, macrophages
- GSE296797 CD38+CD93+ microglia and macrophages drive neuroinflammation through glycolysis-histone lactylation axis in multiple sclerosis model mice 17 samples shares ros, macrophages
- GSE319614 Bulk RNA-seq transcriptome profiling of hippocampus across 17 autism spectrum disorder mouse models 269 samples shares ros
- GSE332646 Bulk RNA-seq of juvenile mouse liver following NDMA or saline treatment in WT and Mgmt-/- genotypes across multiple timepoints 214 samples shares ros
- GSE304862 Semaglutide and exercise synergy in obesity: preserving muscle mass and uncovering organ crosstalk 209 samples shares ros
- GSE229815 Transcriptional profiling of 12 month striatum and cortex from a mouse model of Huntington's disease (Q140) crossed with Msh2 heterozygous or homozygous conditional knockout mice [Msh2_12mon] 138 samples shares ros
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.