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Transcriptome alterations induced by acute and persistent human bocavirus 1 infection in human airway epithelial cells

GSE326404 Homo sapiens Expression profiling by high throughput sequencing 3 samples Submitted 2026/05/31 Platform GPL18573
Summary
Human bocavirus 1 (HBoV1) is a common cause of respiratory tract infection in children. It replicates efficiently in human airway epithelial (HAE) cells and is shed into the airways for months after acute infection. It further persists in B cells of tonsillar germinal centers with unknown consequences. Our aim was to compare how acute and persistent virus infections influence the cells. We performed total RNA sequencing to investigate the cellular transcriptomes of polarized HAE cells infected with HBoV1 at 7, 14 and 30 days post infection, and compared the biological pathways enriched across these different time points. We observed significant activation in the interferon signaling pathway and elevation of pattern recognition receptors, including toll-like receptor 2, only in the acute phase, whereas a slight elevation of the p53-signalling pathway was still observed after 30 days. The results indicate that the cells seem to reach an equilibrium state with the virus where viral activity and cellular impact are balanced.
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Direct links to NCBI, no account and no request form: the whole study as GSE326404_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 3 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1445212 and SRA study SRP687754. Searching any of these in the dataset finder brings you back here.

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