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Ocy454 cell gene expression changes with PTH, FAK inhibitor and PDE8A deletion

GSE326861 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/04/07 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
12 samples: 3 × Ocy454 cells wild type vehicle vs 3 × Ocy454 cells wild type FAKinh vs 3 × Ocy454 cells wild type PTH vs 3 × Ocy454 cells PDE8A KOGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Regulation of cAMP levels in osteocytes by mechano-sensitive focal adhesion kinase and phosphodiesterase 8AiScience · 2026
Summary (written by the submitters)
Cells from the osteocyte cell line Ocy454 were treated with vehicle (DMSO), +/- PTH (100nM), +/- FAK inhibitor (PF431396 10uM) or underwent PDE8A deletion via CRISPR/Cas9. The goal of this study is to determine the similarity between the genes that are differentially expressed with PTH treatment, FAK inhibition and PDE8A deletion and identify common molecular pathways.
Published in
Regulation of cAMP levels in osteocytes by mechano-sensitive focal adhesion kinase and phosphodiesterase 8A
Papaioannou G, Sato T, Houghton C et al. · iScience 2026 · PMID 42231949 · doi:10.1016/j.isci.2026.116049
This dataset
Open GSE326861 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE326861_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1447497 and SRA study SRP688936. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42231949) ↗
Study design
3 × Ocy454 cells wild type vehicle vs 3 × Ocy454 cells wild type FAKinh vs 3 × Ocy454 cells wild type PTH vs 3 × Ocy454 cells PDE8A KO

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.